Adipose Tissue Protocols (Methods in Molecular Biology Vol by Gerard Aihaud

By Gerard Aihaud

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78. , and Pénicaud, L. (1994) Hétérogénéité et plasticité cellulaires des tissus adipeux. Méd. Sci. 11, 1099–1106. 79. , Koza, R. , and Kozak, L. P. (1998) Emergence of brown adipocytes in white fat in mice is under genetic control: effect on body weight and adiposity. J. Clin. Invest. 102, 412–420. Choosing an AT Depot 19 80. , et al. (1995) Adrenergic receptors and fat cells: differential recruitment by physiological amines and homologous regulation. Obesity Res. 3, 507S–514S. 81. , and Nougues, J.

Sample Collection The size of AT samples collected from human and animal subjects for LM study must be sufficient to preserve the greatest possible morphologic detail without being excessive, to avoid embedding problems. In our experience with AT, optimal sample size is about 2 cm3. Because of the texture of this tissue and to its high lipid content, all the parts exhibiting mechanical artifacts (compression and deformation caused by incorrect handling of forceps and knives) must be discarded. To perform this operation, we make a clean cut with a razorblade.

And Bouillaud, R. (1991) Molecular studies of the uncoupling protein. FASEB J. 5, 2237–2242. 19. , et al. (1997) Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nat. Genet. 15, 269–272. 20. , and Giacobino, J. P. (1998) The uncoupling proteins, a review. Eur. J. Endocrinol. 139, 1–9. 21. Lafontan, M. and Berlan, M. (1993) Fat cell adrenergic receptors and the control of white and brown fat cell function. J. Lipid Res. 34, 1057–1091. 22. Giacobino, J. P. (1995) Beta 3-adrenoceptor: an update.

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